Technology
LinkSēq™ | Accurate, Fast, Easy HLA Typing
LinkSēq™ is the first HLA typing chemistry developed for use on real-time PCR platforms (click here). This innovative chemistry, an end-point PCR process, uses allele specific amplification combined with SYBR® Green to detect PCR products on the instrument without electrophoresis or probing steps. Rather than separating DNA based on size, as in gel electrophoresis, melt curves identify specific DNA based on melting temperature (Tm). As double stranded DNA melts, SYBR® Green is released into solution, which causes a change in fluorescence. This change is recorded as a "melt curve". Once the PCR amplification process is completed, a melt curve analysis is performed to identify specific HLA alleles. SureTyper™ software identifies the HLA type by comparing the pattern of positive and negative reactions to known HLA sequences, simplifying the genotyping process. Linkage Biosciences tests are compatible with various plate-based real-time PCR systems.
Products
HLA Molecular Typing Products - both 384-well and 96-well formats available:
HLA-ABCDRDQDP+ (10 tests each) (New DP Plus test)
HLA-ABCDRDQ+ (10 tests each)
HLA-ABCDRDQ (10 or 20 tests each)
HLA-ABDRDQ (10 or 20 tests each)
HLA-ABDR (10 or 20 tests each)
HLA-ABC (10 or 20 tests each)
HLA-ADR (10 tests each)
HLA-A (20 tests each)
HLA-B (10 tests each)
HLA-C (20 tests each)
HLA-DR (20 tests each)
HLA-DQ (40 tests each)
HLA-DP (20 tests each)
HLA-DRDQ (10 tests each)
HLA-DPA1 and DPB1 Now Available!
*Note: DQ and DP tests include alpha and beta loci; HLA-DR tests include DRB1345
Genetic Markers:
Celiac Disease (DQ2.5 and DQ8.1)
HLA-B27
HLA-B*5701
HLA-DQB1*0602 (coming soon)
**Note: Genetic Marker tests are packaged in strip tubes for ease of use. Plate-based format available on request.
Contact the sales department for the latest information.
*Linkage Biosciences™ and LinkSēq™ are trademarks of Linkage Biosciences Inc. SYBR® is a registered trademark of Life Technologies Corporation. This product line is covered by issued and pending patents worldwide. For Research Use Only.